FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835586
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7400.039.1113361
TCGCTCT8700.038.068554
TCTGTCG7550.036.2380148
TCTCGCT9250.033.4762842
CTCGCTC9050.033.4130863
CGCTCTG9650.031.3355885
GTGTTAT7650.030.266551
GTCTTGC14700.029.533051
GTGTTAG11800.027.593381
CTGTCGC10200.027.529119
GTATTAC4950.026.3112621
GTATAAT5950.025.5374031
GTACTAT3709.094947E-1225.4223671
TAATACT4900.024.9793174
TTATACT5800.024.8273144
TTGCTCT20100.024.7161464
TTACTCT9800.023.5099454
GTGTACA8350.023.3965441
GTATAGA6300.022.9701521
GTTCATA5450.022.5697541
Produced by FastQC (version 0.11.5)