FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226890
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTGACGCTGCCGACGATCAAGTGTGTGTAGATCTCGGTGGTCGCCGT33550.15065854173308962Illumina Single End PCR Primer 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC3900.026.4168661
GGTTTAA4800.024.5299471
CTATACT3251.6189006E-924.35984
GTCCTAC3951.8189894E-1124.219441
CTAGACT4403.6379788E-1222.9002254
GTGCTAC3553.4779077E-922.8024851
GTATTGA5350.022.0081771
GTCTTGC16250.021.2844471
GTCTTGA15650.021.1600191
GTGTTAA4251.2223609E-920.7783071
GTCCTAA3207.348099E-720.6971421
GGTTAAT3258.527768E-720.3787251
GTGTAAG6200.020.177861
GTGTAAA7700.020.0699561
GTTCTAA4401.882654E-920.0699541
TGTTACC4052.0369043E-819.5589682
GTCTAGC4903.4015102E-1019.5238341
GTTAATC1850.00526665919.4519273
GTCTAGT5004.456524E-1019.1333581
AACCGTG4955.2386895E-1018.9021997
Produced by FastQC (version 0.11.5)