FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3318115
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.042.7771768
GTCTCGC12700.039.1554871
TCGCTCT12450.037.0111274
GGTATAC6050.033.3541561
CTGTCGC16850.032.473979
TCTCGCT14900.030.9552572
CTCGCTC15350.029.1070253
GTCTTGC26450.028.8820131
GCGTTAC1607.115993E-527.0257991
CGCTCTG16250.026.5839545
GTCTTAC12350.026.2598861
GGGTTTC48750.024.6881831
GTTTCAC44050.024.539173
GTATTAT10350.024.371091
GTATTAC7300.023.6938511
GTATTAG10200.023.3163761
GTGTAAC9050.023.093871
GTCCTAC9750.022.9141831
GTATTGA8900.022.673331
GTGTATA14950.022.6570821
Produced by FastQC (version 0.11.5)