FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054778
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.040.567698
TCGCTCT11900.039.9344024
GTCTCGC12000.034.9743231
TCTCGCT13250.034.2443282
CTCGCTC14400.031.5002563
CTGTCGC15700.029.8125749
CGCTCTG14750.029.7750515
GTCTTGC22350.028.4910011
GTATTAC6450.028.0467681
GTGTTAG17450.027.783181
GTGTAAC8700.026.615421
GTCCTAT7000.025.8430961
GTACCGT2001.12555535E-525.1989676
GTCTTAC10350.024.469791
GTGTTAC12200.024.317931
TTACACT13950.023.7429054
GGGTTTC43750.023.1554151
GTTTCAC38750.023.0401843
GTATTAG9200.022.809341
GTCTCAC30600.022.7013871
Produced by FastQC (version 0.11.5)