FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054778
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11800.038.4248128
GTCTCGC11600.034.88561
GTATTAG9500.029.4307611
GTCTTAC9200.028.790961
CTGTCGC17400.027.7158729
TCGCTCT13650.026.8895554
GTCTCGA25800.025.9423526
CTCGCTC14950.025.0327323
CTCGATC12200.024.7766558
GTCTTGC24400.024.7266641
GTATTAC6850.024.7046531
GGGTTTC39700.024.649191
TCTCGCT15750.024.2324542
CCGTGTT8700.023.9928449
CGCTCTG15350.023.9115585
TCGATCT12950.023.3443899
GTGTTAC10450.023.234811
GTTTCAC36600.023.0065173
GTGTTAG16950.022.5722661
GTATTAT8550.022.3742641
Produced by FastQC (version 0.11.5)