FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716630
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9500.046.9925778
CTGTCGC13700.035.2140059
GTCTCGC11200.034.252751
GTCTTGC21350.032.5471
TCGCTCT11100.032.433984
GTATAAC4650.031.1325471
TCTCGCT13350.026.9760192
TTGCTCT29150.025.4420364
CTCGCTC13400.025.2526133
GGCTAGA14350.024.2117941
GTGTTAC9050.023.1946071
TAATACT8450.023.0070154
CTTGCTC28250.022.6821783
GTGTAAC8950.022.6450121
TTACACT11700.022.1549034
GTCTCAC24350.021.7000891
GGATACG2704.5121105E-621.4468651
GTGTAAG14850.020.9594361
CGCTCTG14800.020.9191475
GTCTCGA21450.020.8114156
Produced by FastQC (version 0.11.5)