FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716630
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT11300.035.0294574
TCTGTCG9050.034.1967668
GTCTTGC21500.033.8733251
GTCTCGC9100.032.3355061
TAGTACT6800.027.5177924
GTATAGT7500.025.5019361
GTGTTAG17200.025.2339151
GTATTAC5550.025.1838251
GTGTTAC9750.024.8983381
CGCTCTG12250.024.6752955
CTCGCTC13250.023.8993423
TCTCGCT13300.023.8222122
GGGTTTC33800.022.199571
GTATACT5650.022.1340831
GTCTAAT7350.022.0189421
CCGTTCG2303.3232107E-521.9078279
TTGCTCT30750.021.7664184
GTATAGG6800.021.636261
GTATTGG7150.021.6059991
CCGTGCG4058.8220986E-1021.3282559
Produced by FastQC (version 0.11.5)