FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3401554
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13050.038.6212358
GTCTTGC26950.037.144151
GTCTCGC13350.033.446051
TCGCTCT13450.031.5836244
GTATTAG10500.030.8643841
TTGCTCT31750.029.0267544
GTGTTAC10550.028.670231
CTGTCGC18450.028.0979569
CTTGCTC32500.027.4707533
GTGTAAA17750.026.3723831
TAATACT10150.026.2462884
GTCTCAC32100.025.5760921
GTCCGTA2001.1248663E-525.2010972
CTCGCTC16300.025.1778983
TGTACGG3151.1223165E-925.1439512
GGGTTTC43500.024.9989681
TTACACT13450.024.6245214
GTACTAG6800.024.3586541
GTATTGC8900.024.2753581
TCTCGCT17350.023.6552072
Produced by FastQC (version 0.11.5)