FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591958
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8700.038.0680058
GTCTCGC9650.033.6144831
TCGCTCT9450.032.7610784
CTGTCGC11050.029.9720929
TCTCGCT11600.028.585192
GTCTTGC20400.026.854971
CTCGCTC12350.025.6799053
CGCTCTG11450.024.5233965
GTGTTAC9800.024.2733151
CGTTTCG2101.6422966E-523.9993939
GTATAGG7150.023.1879861
GGGTACG2552.848561E-622.6147121
TTACGGA2303.3176846E-521.9124894
TAAATCG2303.3176846E-521.9124894
GTATTGC5950.021.8070431
GTGTAAA13200.021.297661
GTGTTAG15600.021.2556551
GTCTCAC26150.021.2256151
TACACTG13400.020.9546955
GTTTCAC33100.020.9050373
Produced by FastQC (version 0.11.5)