FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247841
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT8750.031.2676944
GTCTCGC9250.031.1501981
GTATTAC4950.029.1049821
TCTGTCG8300.028.62578
GTGTTAG12000.028.213641
GTCTTGC16900.027.7057021
TCTCGCT10250.027.4083752
GTTAGCC9150.026.7646583
GTCTTAC8100.026.6795671
GGGTACG1959.209605E-625.8586561
CTCGCTC10600.025.1419943
GTGTAAG9550.024.8916151
GTCTAGC8150.023.86431
GTTTCAC25800.023.7302933
TTCACCG5200.023.5378025
GTACTAG4900.023.5215761
GTATTAT5550.023.3626481
GTATAGA7600.022.7478391
GGGTTTC28250.022.4391671
GGTCTAC4505.456968E-1222.4108351
Produced by FastQC (version 0.11.5)