FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247841
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8650.043.28498
TCGCTCT8900.041.258184
GTCTCGC8250.041.0252341
CTCGCTC10500.035.656933
GTCTTGC17400.035.1784631
CTGTCGC11100.033.082359
CGCTCTG11050.032.5789875
GTCTCAC21450.032.2293171
TCTCGCT10800.032.000522
GGTATAC4050.030.2274171
GTACTAC3400.029.6521571
GTGTTAC8800.028.6412891
GTGTAAC6000.027.6047481
TGCTACG2405.6432327E-827.0004392
GTGTTAG12050.025.6973631
TTGCTCT22950.025.4116124
TATACTG6350.024.944735
CTGTTGC19500.024.7394129
GTATAGC4950.024.7315251
GTCTTAC6700.023.6458551
Produced by FastQC (version 0.11.5)