FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943379
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9750.042.8300978
GTCTCGC10400.038.7752081
GTCTTGC21450.036.9287721
CTGTCGC13700.033.634519
TCGCTCT11800.031.7283154
GTACCGT1952.769575E-729.5430151
TCTCGCT13500.028.2686212
GTCTCAC24400.026.2663441
CTCGCTC14550.026.2263933
GGGTTTC37050.026.04451
TTGCTCT29800.025.6103384
GTTTCAC35150.025.1944853
GTATTAC7250.024.8314151
GTCTTAC9400.024.5144181
GTGTTAG15600.024.465311
GTGTTAA9450.024.384711
GTATAAC7150.024.1715581
GTGTTAC8100.024.00371
GTTCTAG11450.023.8988821
TCTCGAA18100.023.8670277
Produced by FastQC (version 0.11.5)