FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3004809
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10450.036.515748
GTCTCGC11600.034.16941
GTCTTGC23100.029.3257061
GTATTAT9450.027.4538541
CTGTCGC15100.027.1780579
TCGCTCT12500.027.0712534
GTCTTAC10950.025.6674731
CTCGCTC13350.024.8297863
GTCTCAC28750.024.3145641
GTGTTAG19200.024.0221211
TCTCGCT14550.023.7740752
GTATTAA8800.023.7491441
GTATTGG9450.023.6408181
GTGTTAC10750.023.4634691
TTGCTCT31350.022.7362144
GTGTAAA16350.022.4794181
GTCCTAC7800.022.1742671
GTACTAA6850.022.0933381
CGCTCTG14700.022.0402055
GTGTAAC8750.021.4140051
Produced by FastQC (version 0.11.5)