FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3004809
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11450.034.585918
TCGCTCT11250.034.5601844
GTCTCGC12100.032.731261
GTCTTGC22700.030.1358281
GGTATAC5650.028.0388131
GTATTAT7850.027.5192761
TATACTG7850.027.5160685
CTCGCTC14550.026.7217923
GTCTTAC9400.026.0457251
GTGTTAC10000.025.9231571
GTGTTAG17300.025.3903751
CGCTCTG14500.025.3242745
TCTCGCT16100.024.5972212
CTGTCGC16250.023.9262819
GGCTAGA15750.022.8599281
GTATAAT9000.022.4027291
GTATTAG8050.022.362971
GAGTGCA55200.022.3048386
GTCCAAT6200.022.0672041
TTACACT12450.021.976024
Produced by FastQC (version 0.11.5)