FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566452
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13150.044.8963248
CTGTCGC16800.038.1419949
GTCTCGC14750.036.643761
GTCTTGC29200.036.0330351
TCGCTCT14750.033.192474
GGTATAC6050.029.7793661
CTCGCTC17500.028.8248083
GTATTAG10600.028.554481
TCTCGCT17800.027.5308552
TTGCTCT41750.026.9023864
CGCTCTG17400.026.4821595
CCGTATA1400.001035609625.7378792
GGGTTTC48450.025.4350831
GTTTCAC44950.024.2077083
GTCTTAC10550.023.9081731
CTTGCTC38100.023.8315333
GTACTAC5800.023.607851
GTGTTAC12600.023.450071
GTGTTAG21800.022.4793221
ACCATGT39350.021.5903648
Produced by FastQC (version 0.11.5)