FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792960
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5650.040.7749638
GTCTCGC7200.032.9978451
TCTCGCT7100.032.4485862
GTCTTGC15650.031.2823031
CTCGCTC8500.030.4921283
TCGCTCT8100.030.2194084
GTATATA6200.029.0303631
GTATTAT4500.028.7981191
TAATACT4800.028.4973474
GCGTAAT1306.755302E-427.69051
ATACCGC1607.16514E-526.9974866
CGCTCTG8800.026.1793775
GTCCAAT4300.025.1146371
TTTGCGC1450.001277806424.8259663
GTCCAAC4350.024.8259661
ATAATAC5050.024.2360423
GTCTCAC17750.023.9308341
GTGTTAG10650.023.6604271
TAACACT8000.023.397824
TTACACT7700.023.3744454
Produced by FastQC (version 0.11.5)