FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753014
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6350.039.6840558
GTCTTGC13250.033.7081
GTATTAG5950.032.6891751
GTATTAT4550.031.6647281
GTCTCGC7450.029.9752351
GTGTTAC7050.029.6323451
CTATCGT1000.006284484628.8001062
GTATAAT4550.028.4982551
TTGCTCT17150.027.708564
GGGTTTC22850.027.1124921
TCGCTCT8750.026.3315264
ATACTTT6200.025.5477566
TTAGACT5400.025.333434
GTATAAA6600.025.1038911
CTTGCTC17800.025.0787453
GTTTCAC20200.024.950593
GAGTTAC4700.024.5233211
TCTCGCT9700.024.4949382
CTCGCTC9300.024.000093
CTAGCGC1500.001556148324.000093
Produced by FastQC (version 0.11.5)