FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785145
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6150.036.2896848
ACTTGCG1352.248248E-531.9973558
GTCTTGC13550.030.8166391
GTCTCGC6400.030.3724881
GTATAAA6650.029.2306671
GGGTTTC25750.027.9588551
GTACTAT2853.5106495E-1027.7871761
ACCGCTA1050.00797805527.4263068
GTTTCAC23750.027.2819563
GTCTCAC16300.025.6175161
GTTAGCC8750.025.5064623
GTGTTAG12150.025.4793741
GTACAGA8900.025.0765781
TTGCTCT17250.024.623364
ACCGTGT6200.024.385088
GTATTAG5650.024.2103861
TCGCTCT8050.024.1463954
GTATACG1500.001556942623.9980161
ACTATGT6900.023.9980148
GTATAAT5150.023.7650261
Produced by FastQC (version 0.11.5)