FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785145
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7500.036.509641
GTACCGT1001.4428011E-436.0292551
TAATCCG800.002108293135.9969645
TACTCCG1301.7367945E-533.2279665
TCTGTCG7650.032.9383938
GTCTTGC12600.030.3103241
GGGTTAC4550.030.0903661
TCGCTCT8100.029.33254
CGCTCTG9400.027.5721425
GTCTTAC5300.025.8322961
CTCGATC6500.025.4747738
GGTTACG1701.0754595E-425.4324151
GTTTCAC21650.025.2741073
TAATACT4350.024.826884
TATACTT4650.024.7721025
GTACAAT5300.024.4727021
CTCGCTC9900.023.9993173
GGGTTTC22350.023.85831
TCTCGCT10000.023.7593232
TCGAACT9300.023.2238469
Produced by FastQC (version 0.11.5)