FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354536
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAC450.009890038548.020871
TCTGTCG5800.042.2034238
GTCTTGC10700.037.0254361
GCTTACG850.002837076833.8820652
TCGCTCT6100.033.04894
GTATTAA3750.032.654191
GTACTAA2705.456968E-1232.0139121
GTCTCGC6000.028.8125211
TCATTCG1504.612715E-528.7997574
GGATAAC3301.8189894E-1228.3759671
GGGTAAT4100.028.1097761
TAATACG1050.007974527.4283394
TTCACCG3950.027.3395215
GTGTTAG7950.027.1816231
TTGCTCT14050.027.159914
CGCTCTG6100.027.1453085
CTGTCGC9650.026.8578939
TTACGCC1358.418057E-426.6664433
GTCTAAT3555.456968E-1226.3776571
GTTATAA2207.925373E-726.1932031
Produced by FastQC (version 0.11.5)