FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506991
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5200.037.3822828
GTCTCGC5000.035.9977531
GTCTTGC10500.032.226561
GCGCTAG1453.6619866E-529.7912431
TTCACCG4200.029.1410395
GTGTAAC4000.028.7982041
GTTTCAC18000.028.79823
TTACTCT7250.028.79824
GTGTTAC4250.028.7981991
GTATTGC3800.028.4192771
GTATTAA3600.027.9982511
GTATTAC3103.092282E-1127.8692281
CCGTTTA1555.7703353E-527.8692289
ATTCGAC1306.754687E-427.6905783
GGGTTTC19250.027.302191
GTATTAT4550.026.899421
CTGTTGC13150.026.827229
GTATTGG4900.026.4473291
TATACTG3900.025.8445385
TATTAAC3358.185452E-1125.7894342
Produced by FastQC (version 0.11.5)