FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506991
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC5950.038.7425081
ATTCCGA1001.4500521E-435.9981086
TCGCTCT6450.031.2552094
GTCTTGC10300.030.7730241
CGCTCTG7100.030.4209385
TCTGTCG6800.029.6455028
CTCGATC6100.029.5066478
GTGTTAC5800.028.5663381
GTCTCGA12950.027.7977686
ACGTTTA1306.7542976E-427.6908536
TCGATCT6350.027.211179
TCTCGCT8400.026.5709132
GTATTAG4450.025.900891
CTCGCTC7950.025.3579983
TTACTCT6650.024.9017734
TCTCGAT7250.024.8262837
TAATACT3001.8391802E-823.9995354
GTATAGT4900.023.5222381
TTGCTCT15400.023.3761714
GTGTTAG9000.023.2118741
Produced by FastQC (version 0.11.5)