FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593150
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT10750.037.506544
GTCTCGC10850.033.867741
TCTGTCG9700.031.1742958
TATACTG8350.031.0409565
GTATTAC4900.030.879411
GTCCTAC5950.027.8520151
TCTCGCT13450.027.8361072
CTCGCTC13050.027.5858863
CGCTCTG13200.027.2718835
GTCTTGC19700.027.06521
GTCTTAC9600.027.0194821
GTGTTAC8600.026.810031
GTATTAA6000.026.4190521
CTATACT5500.026.1815134
GTATTAT7450.024.1785071
GTCTCAC23950.023.4657731
GTGTTAA8950.022.5413931
GTTTCAC30400.022.4997373
GGCTAGA12850.022.4286251
TCACTCT27650.022.3939534
Produced by FastQC (version 0.11.5)