FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121692
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT9500.034.8621184
GTCTCGC8950.031.3955611
TTACACT8650.028.299734
GTCTTGC16500.027.9461941
TCTGTCG8850.027.6595368
TTATACT6300.027.4277524
TACACTG11000.025.5259065
GGTATAC4700.024.5272461
CGCTCTG11750.024.5093275
GTCCTAC5000.024.4965881
GTATTAC5050.024.2540471
CTCGCTC11600.024.2061733
GTGTACG2701.7533239E-724.016261
ACCATGT21950.023.2880468
GGGTAAT6550.023.099611
TCTCGCT11850.023.0879172
GTGTAAG9450.022.872631
GTAATAC5050.022.81123
GTGTAAC6650.022.7522471
GGCTAGT6650.022.7522471
Produced by FastQC (version 0.11.5)