FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3795716
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11900.047.7976958
GTCTTGC29500.038.3413731
GTCTCGC15400.037.4247861
CTGTCGC17450.033.8333669
GTATAAC6900.031.3229181
TCGCTCT17000.030.0701964
TCTCGCT18900.029.7315222
CTCGCTC20450.027.4780333
CGCTCTG18150.026.9748545
CTACGCA3057.730705E-1025.9668719
GTGCTAC11500.025.0583361
TTGCTCT40550.024.147654
GTCTCGA31050.023.4199856
TCTCGAT14200.023.3236377
GTATTAG12750.023.1666761
GTCTCAC37650.023.1531681
GTCGACC2802.508532E-723.1425538
TACACTG20500.022.828975
GTGTTAG23850.022.6549421
GGGTTTC49000.022.6419941
Produced by FastQC (version 0.11.5)