FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513039
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11900.042.380691
TCTGTCG11000.038.6176348
TCGCTCT14000.034.9709324
GTCTTGC26350.032.2640081
TCTCGCT14700.031.3665812
CTCGCTC15200.029.8608043
GGGTACG3500.028.818871
CTGTCGC15900.028.5278979
CGCTCTG16500.028.3632325
GTCGTAC1306.7282154E-427.7104511
GTCTCAC31350.026.428791
GTCTTAC9900.026.1989691
TTACACT14450.025.9096664
TTGCTCT36150.025.8917474
GTGTTAG20900.025.16481
TACACTG18900.024.761555
GTATTCG1751.3112073E-424.7018891
TCTGTCA34200.023.9996598
GTGTTAC12300.023.4299741
CGGTACT1851.9200887E-423.3510174
Produced by FastQC (version 0.11.5)