FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3281268
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG1204.2280697E-429.9999435
GTCTCGC12300.029.8586061
GTCTTGC26000.029.3587111
TCTGTCG16050.027.8130328
TCGCTCT13500.026.1332844
GTATTGG11250.024.9641361
GTGTTAC12250.024.1019521
CTCGCTC15600.023.9999543
GTCTCAC33950.022.9080681
GTACTGG12600.022.8609311
TTGCTCT36300.022.8098754
GGGTTTC45750.022.666051
GTATAAG9000.022.4037131
GTTTCAC41300.021.966063
TACACTG17700.021.966065
CTGTCGC22350.021.9069
CTTGCTC35350.021.7934533
GTACTGC11000.021.603581
CGCTCTG14700.021.550985
GTCTTAC12450.021.4011361
Produced by FastQC (version 0.11.5)