FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3991856
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15750.038.3974721
TCTGTCG13600.037.567878
TCGCTCT15700.035.754014
GTCTTGC29200.031.8061281
TCTCGCT18400.030.1280732
CTCGCTC18800.028.7211573
CGCTCTG18450.028.0833975
CTGTCGC19850.027.9143419
GTCTCAC36750.024.292281
GTGTTAG25850.023.1164671
GTCTTAC13400.023.1029591
TTGCTCT41100.022.7630944
CCTATCG1902.3085068E-422.7245258
GTGTAAC9300.022.4501361
CTTGCTC39150.022.2511313
TACACTG19300.021.9992665
GTCCTAC10000.021.598581
GTATAAG14050.021.5217151
GTACTAA7750.021.3663351
GTACCGT2053.8577418E-421.0625466
Produced by FastQC (version 0.11.5)