FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3991856
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15500.036.701091
GTCTTGC28050.034.1430551
TCGCTCT16200.033.7775234
TCTGTCG17600.030.2724978
CGCTACG1952.7739225E-729.5382372
CGCTCTG17850.029.4451565
GTACTAC6350.028.3497921
CTCGCTC18700.027.7217163
GTGTTAG21500.026.458931
TCTCGCT20150.025.3695342
TACGCCG2202.353706E-522.9089165
GTCTCAC36650.022.7912241
CTTGCTC39100.022.6494453
GTCCTAC10300.022.3715651
TTGCTCT42050.021.91664
TGTTAGC20050.021.9050712
TAATACT10850.021.898454
GTATTAA12200.021.8386361
TTACACT16900.021.7276464
GTGTAAC11050.021.5047931
Produced by FastQC (version 0.11.5)