FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7086912
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC28150.039.8925551
GTCTTGC54200.034.9302831
TCGCTCT30950.034.6730464
CGCTCTG32650.028.8971185
CTCGCTC35350.028.7273853
TCTCGCT37750.028.4285092
TCTGTCG34050.027.0792058
TTGCTCT70200.025.5463354
GTGTTAG42300.025.526811
GTATTAA19800.024.7223281
GTCTCAC64650.023.828181
GTTAGCC34200.023.7968083
CTTGCTC72050.022.6912423
TATACTG20100.022.5741215
GGAGTGC139700.022.5294935
TTACACT30400.022.5069394
GAGTGCA153950.022.315426
GTATAGG17400.021.926651
GTATTAG21450.021.813821
GTGTAAT26850.021.7163281
Produced by FastQC (version 0.11.5)