FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7170440
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG23400.044.3009078
GTCTCGC27200.034.9433751
CTGTCGC31300.034.4995049
GTCTTGC55950.033.0744671
TCGCTCT27650.032.807394
TCTCGCT32150.027.771582
CTCGCTC31850.026.2245713
GTCTCAC66250.024.6717411
CGCTCTG33000.024.6524875
GTATTAG22750.023.4212551
TTGCTCT73750.023.0380764
GTTCTAC19700.023.026831
GTCTTAC22800.022.7382741
CTTGCTC68700.022.534173
GGAGTGC147650.022.2345125
GTGTTAG45550.022.130921
TCTGTCA75650.021.7917698
GAGTGCA161650.021.7325176
CTGTTGC62350.021.4754569
GTATTAC15900.021.2843591
Produced by FastQC (version 0.11.5)