FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751859
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT55100.20022828204497395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG6550.032.9776271
ACTTACT1000.00628472128.8004632
GTGCTAC3052.5465852E-1128.3283231
TATAAGA6750.026.6670912
GTTCTAA1400.001041007925.7146971
GTCTTAC3109.313226E-1025.5487981
TGTATAA4350.024.8279842
GCTAAAC1450.001277374824.8279842
GTGTATA5100.022.5828519
ACTGTGC3956.4574124E-1021.8682028
TAAGAGA8600.021.7638364
GAACTTA1750.003815334520.5665256
GTTCTAC7550.020.0268121
TAAACCC3301.2195578E-619.633114
CCCTTAA1850.00525470919.459771
GGGTAAC6350.019.2758981
TCTATAA2659.866516E-519.0191732
TGTGTAT6600.018.5410358
CGTATAT2000.00824103618.0002881
GTCCTAC3602.7986298E-618.0002881
Produced by FastQC (version 0.11.5)