FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362179
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8050.038.453948
GTCTCGC8250.034.034931
GTCTTGC18300.032.2609371
GTATTAC6800.031.763371
CTGTCGC10600.031.2406629
TCGCTCT8550.031.1552684
GTCTCAC20050.028.726971
GTCTTAC7500.025.918911
TCTCGCT10050.025.789962
GTACAGA10400.025.614311
GTACCGG2001.1254231E-525.1989421
CTCGCTC10600.025.1310183
GAATAAC6350.024.9438341
GTGTTAC7050.024.509611
TAACACT9850.024.1197934
GTGTAAA12900.023.9989911
GTGTTAG14450.023.9159491
CGCTCTG11150.023.8903625
GTCTAAG6850.023.1231161
GTATTAG8450.023.004951
Produced by FastQC (version 0.11.5)