FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362179
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8400.038.562311
GTACGGA1604.8928996E-835.991491
TCGCTCT10350.033.3996734
TCTGTCG10450.029.6398728
GTGTTAG14400.029.4930251
GTTCTAC6000.028.793191
GTCTTGC16200.028.437721
GTTAGCC10300.027.9681763
GTATTAC5300.027.1633871
TCCGCGA1607.140064E-527.0119198
CGTAGTG2757.2741386E-926.1883812
GTCTTAC7700.026.175631
CTCGCTC11700.025.8526333
GTCCTAG10150.025.5309071
CGCTCTG11650.025.3454065
GTACTAG4550.025.3126961
GTCTCAC22400.024.7441481
GGAGTGC43950.024.7434025
TGTTAGC13400.024.7226142
GTATTGC5300.024.4470481
Produced by FastQC (version 0.11.5)