FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431041
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT8800.033.5414434
TCTGTCG7150.031.2111248
GTCTTGC19950.030.3177781
GTCTCGC8800.028.638241
GTCTTAC8000.026.1017131
GTCTAGC8300.026.0258031
TACACCG2259.69374E-725.596945
CTCGCTC10150.024.8292143
GTCTCAC22050.024.8179551
TCTCGCT10200.024.7075022
GTGTAAG12250.024.6873321
CGCTCTG10250.024.5824285
GGCTAGA12000.023.4015331
GTACGGG2202.3518856E-522.9105931
CTGTCGC9250.022.5688829
GTATTAG8700.022.3462921
GTCTAGA11000.022.2560061
GGTATAC4900.022.0422611
TACACTG11600.021.721545
GTACCGG2003.2520364E-421.6014181
Produced by FastQC (version 0.11.5)