FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431041
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11300.033.7810828
GTCTCGC9700.033.3931731
GTCTTGC18100.030.6216851
TCGCTCT10500.030.1745764
GTATTAA6250.029.9440331
GTACTAG4450.027.49831
GTATTAC5800.027.303081
CTCGCTC11400.027.1607283
GTCTCAC24300.024.586051
CGCTCTG11250.024.3225365
TCTCGCT12750.024.2853872
TAGTACT5050.024.2401524
ATACCCG2401.6974045E-624.0025044
GTCCTAC5700.022.7307931
GTCTAGT9600.022.4940151
GGCTAGT7050.022.4621091
GGAGTGC47800.022.4458565
GAGTGCA50650.022.178036
TACCCGT1952.739694E-422.1561575
CGTTTCG2603.4039258E-622.1552459
Produced by FastQC (version 0.11.5)