FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783259
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.044.150418
TCGCTCT11100.032.4326254
CTGTCGC14900.032.3754469
GTCTCGC10800.030.6916581
GTCTTGC23150.029.8817841
GGCGTAC1400.00103617225.7352431
GTATAAT7300.025.6647341
GTGTTAA10850.025.237141
CTCGCTC13150.025.196873
CGCTCTG13400.024.716125
GTACAAG10000.024.499951
GTCTCAC25750.024.0661981
GTGTATA15050.023.939761
GTCTTAC9050.023.8868541
GTGTTAG15550.023.6333921
GTATTAG9550.023.3907761
TCTCGCT13450.023.0290662
GTGTAGA14000.022.6470131
TTGCTCT30150.022.4478954
GTATTGG8450.022.17191
Produced by FastQC (version 0.11.5)