FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783259
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9850.036.548221
TCTGTCG13150.033.3992428
GTCTTGC19850.030.4685131
TCGCTCT11650.028.4291844
GTCTTAC8450.027.2662721
GTGTTAG17550.025.025641
TAATACT7300.024.6575364
GTCTAGC11100.024.648651
GTATTAG7400.024.3243241
GGTATAC5650.024.2123871
CTCGCTC12550.024.0956173
GTACTAT5700.024.0000021
GTGTAAG11100.024.01
GTATTAC5100.023.9999981
GTCTCAC24700.023.3198381
CTAGCCG1550.001883614923.2258079
TCTCGCT14450.022.9204162
GTCGTAA1902.2998112E-422.7368411
TTGCGCG1902.2998112E-422.7368419
CTGTCGC21700.022.5622129
Produced by FastQC (version 0.11.5)