FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3201309
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10450.033.7602238
GTCTCGC12100.033.34731
TCGCTCT11250.031.3599854
CTCGCTC12850.026.9062773
TCTCGCT14150.025.9618722
CGCTCTG13250.024.9958255
CTGTCGC15250.024.5504269
GTATAAC5050.024.25581
GTATAAG8850.023.610911
GTCTTGC25050.022.7236121
GTATAGG9350.022.3482951
GTATTAG9700.022.2847391
GTCTCAC30450.022.0066361
GTACTAG5650.021.679961
GTATTAA7350.021.567181
TATACTT8350.021.5565415
TAATACT10050.021.4925294
GTGTTAG18500.021.0319731
GTGTATA14700.020.5868551
TTACACT15400.020.571424
Produced by FastQC (version 0.11.5)