FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3201309
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12300.031.0252021
GTCTTGC27250.028.8007531
GTACTAG5550.027.2439541
TCGCTCT14150.024.9339054
CTCGCTC15100.023.8420563
GTATAAA11200.023.7863371
TCTGTCG15750.023.772428
GTATTAA8500.022.8711851
CGCTCTG15750.022.8580975
GTACTGA8900.022.6522771
GTATTAG9400.022.2133461
TCTCGCT18750.021.5048982
GTCTCAC30300.021.3866981
TTGCTCT35250.021.0391774
GTATAGG7600.020.8426511
CTTGCTC33500.020.2038293
TACACTG17500.020.1608415
GTGCTAG16700.019.4017071
GTACTAA5950.019.361851
GGTATAC5607.2759576E-1219.2862191
Produced by FastQC (version 0.11.5)