FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870410
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10300.032.163091
TCGCTCT10400.027.6917464
CGGTACT1658.83113E-526.1812844
GTCTTGC22750.024.6916641
GTATAAG7700.022.4469681
GTCTCAC29400.021.801171
CTCGCTC12600.021.7146023
GCGTAGT2353.9085306E-521.4519791
TCTCGCT14000.021.086022
GGAGTGC52400.020.6106285
GTCTAGT11550.020.5763851
GTGTAAG12600.020.5763851
GTGTTAC8800.020.4594751
GTATTAT11450.020.1271211
GTATTAG8600.020.0978661
CTAGCGT2155.300387E-420.0933153
GTCTAGC10400.020.0817621
GTATTAA7900.020.0554661
GTACTAT5750.020.0396121
GAGTGCA57600.019.8749436
Produced by FastQC (version 0.11.5)