FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047002
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10800.036.666288
GTATTAA8950.029.7850861
TCGCTCT13800.029.7397944
GTCTCGC13300.027.0856061
GTCTTGC24600.026.0660421
GTATTAG8300.026.041341
CTGTCGC16650.025.5132439
CTCGCTC15100.024.8042073
GTCTAGA12600.024.0159031
GTATTAC7300.023.686921
TCTCGCT15350.023.4625322
GTATAGA8500.022.8857421
CGCTCTG14900.022.7111685
GTACTAC5100.022.6032031
GTGTTAG17000.022.0381221
TTACACT14600.021.2059524
GCGTTAC2053.8271837E-421.0871351
GTACTAA6500.021.06011
TTGCTCT36100.020.9422954
GGTATAC5002.7284841E-1120.1733591
Produced by FastQC (version 0.11.5)