FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247424
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12500.031.679688
GTCTTGC26050.029.595011
GTCTCGC12900.027.9269051
TCGCTCT13700.026.2771054
CTGTCGC16500.025.3088349
GTATTAA9250.024.9258941
GTGTAAA18200.024.5449851
GTATAGC7150.023.1773761
TCTCGCT15050.022.4938262
CTCGCTC14900.022.2354953
CCTAGCG2704.7089998E-621.341662
GTATAAC6550.020.900411
CCGATAG1750.003810439520.571229
GGTATAC6000.020.4145661
GTATTAC7550.020.040791
TGTACCG2206.1895465E-419.6361665
GTCCTAC8550.019.3822521
GTATAAA14650.019.1809221
GAGTGCA65900.019.1196866
GTCTTAC10600.019.0324481
Produced by FastQC (version 0.11.5)