FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3429512
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14100.033.7253651
GTCTTGC26150.030.858741
TCGCTCT14700.030.8568134
TCTGTCG12400.030.7738638
GTCTTAC12400.027.8901861
TCTCGCT17150.026.0387442
GTATAAG10250.026.0081671
CTCGCTC16200.024.8976963
GTGTAAA17450.024.3606261
GTATTAT9850.024.138461
CGCTCTG16350.023.779565
GTGTAAC9450.023.6353341
GTGTTAC12900.023.4580271
CTGTCGC18650.022.391189
GTATAAC6500.022.1691251
GTACCGA2603.386156E-622.1691231
GGAGTGC62400.022.0382255
GTCTCAC32300.021.8602681
GAGTGCA68300.020.8723966
TTGCTCT40300.020.7243444
Produced by FastQC (version 0.11.5)