FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3429512
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12850.037.542291
TCGCTCT13700.033.636324
CTCGCTC14900.030.4441153
GTCTTGC26600.028.6928231
GTACTAA6950.025.9002691
GTGTAAC9150.025.1812881
TCTGTCG14500.024.8285338
TCTCGCT19050.024.567872
GTACTAT7150.024.1687551
GTGTTAC10850.023.8903121
CGCTCTG16550.023.4933435
GTATTAA9850.023.3917581
GTATATA12800.023.063381
GTATTAG9850.022.6607651
GGAGTGC59850.022.136185
GTCTAAG9800.022.0416581
GGTATAC6600.021.8190141
GTGTAAG14850.021.8190141
GCGTTAC2704.7235135E-621.3341481
GTCTAAC7450.021.2625561
Produced by FastQC (version 0.11.5)