FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493309
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC19550.032.7956661
GTCTCGC9600.031.5174641
TCTGTCG8800.030.2725038
GTATTAA5600.028.3013971
GTACGGA2207.9215897E-726.1963331
TCGCTCT10350.025.7389414
GTATAGA6750.025.6141951
GTATTAG7600.023.697341
GTCTAGT9050.023.0846161
GGCTAGA12300.022.2562351
GTGTAAA14200.021.8149051
CTGTCGC12550.021.8006369
TTGCTCT27100.021.7858164
TCTCGCT11400.021.4795552
GTACCGA1700.00322091321.1763156
CGCTCTG12000.020.399855
GTTCAGC13500.020.2779051
CTTGCTC25800.020.0973073
CTCGCTC12700.019.8467523
ATTACAC9800.019.8409653
Produced by FastQC (version 0.11.5)