FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493309
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10950.030.9041081
TCGCTCT12400.024.9677434
GTATTGG6950.024.863311
GTATAAT5150.023.766991
GTGTTAC7700.023.3766231
GTCTTGC17350.022.8242071
GTACTAT4800.022.51
CTCGCTC12850.022.412453
GTACCGG2603.4058503E-622.1538471
GTATAGA7150.022.1538471
TCTGTCG11200.021.8571438
GTCTTAC8250.021.8181821
GTACTAA4659.094947E-1221.677421
GTATTAT6400.021.3751
GGTCTAC4751.2732926E-1121.221051
CGCTCTG12900.021.2093035
GTTCATA7150.021.1468541
TCTCGCT15400.021.0389612
GTGTACG3458.085772E-820.8695661
GTCTTCG3809.811629E-920.8421041
Produced by FastQC (version 0.11.5)