FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681659
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10900.030.3930361
TCGCTCT11800.027.4574284
GTCTTGC20250.027.029081
TCTGTCG11750.025.1232228
CTCGCTC13100.023.6334173
GGAGTGC47050.022.4950545
GTATAGG5800.022.35051
TACCCGT1952.7418652E-422.1536855
GAGTGCA52900.021.3684656
GTCTCAC24100.021.2170031
GTGTAAA17400.020.6949081
CGCTCTG12200.020.6555885
GCCCGTA2104.523291E-420.571287
GTATACG2155.2917196E-420.0981251
GGCTAGA12900.020.0981221
TCTCGCT14850.019.8786432
GTCTTAG9800.019.841771
GTATTAG9100.019.785241
GGGTACG2557.329225E-519.7697221
TAGACCG1850.00525538119.459325
Produced by FastQC (version 0.11.5)