FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360692
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12500.036.2996671
TCGCTCT14850.029.5755834
GTCTTGC25800.029.0325891
CGCTCTG15400.027.1167245
CTCGCTC16550.024.3623963
GTCTCAC32650.023.823891
TCTGTCG16650.023.7836448
TCTCGCT18800.023.744542
GTATTAC6100.022.4334411
GGAGTGC61500.021.8925535
GAGTGCA65900.021.632656
GTATTAA9400.021.4537031
GTCTAGT13000.021.052921
GTCTAGA14850.020.8551861
GGCTGGA90100.020.7835941
GTCTTAC10500.020.5780431
GTACTAG5303.6379788E-1220.383911
GTCTAAT8500.020.3359491
TTGCTCT37000.020.0431274
CTGGAGT75350.019.6840253
Produced by FastQC (version 0.11.5)