FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158222
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8250.041.01578
GTCTCGC8900.032.3838541
TCTCGCT9700.031.1950552
GTCTTGC17450.030.9687981
TCGCTCT9150.030.708014
CTGTCGC11300.029.9450939
CTCGCTC10050.028.6749133
GTACTAG4300.028.486491
CGTAACT1050.00795053927.4459883
TACACCG1607.1574344E-527.0021215
TGTACCG1701.0809371E-425.4137615
TACACTG12950.025.0212695
CGCTCTG10950.024.3306775
GTCTTAC7500.024.0180241
GTATTAC5400.024.0180231
CGTAACC1500.001550556924.015244
TTACACG1851.9113305E-423.3661794
GGAGTGC39500.022.7865985
TTACTCT10850.022.576544
GTACTAC4154.9112714E-1122.5711561
Produced by FastQC (version 0.11.5)